What does EDTA stand for in phlebotomy?


Ethylenediamine tetraacetic acid (EDTA) is a polyprotic acid containing four carboxylic acid groups and two amine groups with lone-pair electrons that chelate calcium and several other metal ions.

Herein, what is EDTA in phlebotomy?

Tubes with purple or lavender-colored stoppers contain EDTA (ethylenediaminetetraacetic acid) additive that binds calcium ions, effectively blocking coagulation cascade. Clinical laboratories use these to test for whole blood.

One may also ask, why EDTA is not used for coagulation?

 Besides Calcium, EDTA also binds other divalent metal ions like copper quite strongly. Factor VIII and factor V are copper-binding proteins (similarity with ceruloplasmin) and dissociate (=inactivation) if the metal ion is removed.

Similarly, you may ask, what is the full meaning of EDTA bottle?

Ethylenediaminetetraacetic acid (EDTA), also known by several other names, is a chemical used for both industrial and medical purposes. It is an aminopolycarboxylic acid and a colourless, water-soluble solid. Its conjugate base is ethylenediaminetetraacetate. It is widely used to dissolve limescale.

What is the use of EDTA?

Edetate disodium (EDTA) is a chelating (KEE-late-ing) agent. A chelating agent is capable of removing a heavy metal, such as lead or mercury, from the blood. EDTA is used to lower blood levels of calcium when they have become dangerously high.


What type of ligand is EDTA?

EDTA is a hexadentate ligand, which means that it binds six times. It binds twice at the nitrogens and four at the oxygens. EDTA is used most commonly as salts and in a dry form. EDTA is a great chelating agent, allowing multiple bindings in a coordination complex.


How long does blood last in EDTA tube?

It can be stored for 12, 24 or 36 h prior to processing at 4°C and it can be frozen at −80°C for 20 days and then thawed under controlled conditions.


What is EDTA sample?

BACKGROUND: Potassium ethylenediaminetetraacetic acid (EDTA) is a sample tube anticoagulant used for many laboratory analyses. Gross potassium EDTA contamination of blood samples is easily recognised by marked hyperkalaemia and hypocalcaemia.


What does EDTA stand for?

Ethylenediamine tetraacetic acid


What does SST mean in phlebotomy?

Serum separator tube


Why is EDTA used as an anticoagulant?

Ethylenediaminetetraacetic acid (EDTA) strongly and irreversibly chelates (binds) calcium ions, preventing blood from clotting. Citrate is in liquid form in the tube and is used for coagulation tests, as well as in blood transfusion bags.


How much EDTA is in a tube?

A. The nominal EDTA concentration is 1.8mg EDTA per millilitre of blood.


What is the difference between k3 EDTA and k2 EDTA?

Dipotassium EDTA and tripotassium EDTA; that is the only difference. However, when you refer to PCR, I believe that you are talking about the low concentration present in the enzyme (0.1mM) (0.1mM). At such minuscule concentrations, K2 and K3 have no significant difference.


Does EDTA cause cancer?

Summary Though research is limited, scientific evidence currently does not suggest that calcium disodium EDTA has cancer-causing effects.


What is the structure of EDTA?



What is the difference between EDTA and disodium EDTA?

Disodium EDTA has a molecular weight of 336.2 g/mol. The differences are the solubility and the pH of the resulting solution. Although If you will add NaOH to your buffer, the chemical composition of the resulting buffer will be the same in case of using both.


What blood tube colours are for which test?

Green top tube with sodium or lithium heparin: used for plasma or whole blood determinations. EDTA tubes: includes Lavender top, Pink top (used for blood bank testing), Tan top (used for lead testing), and Royal Blue top with EDTA (used for trace metal whole blood or plasma determinations) (used for trace metal whole blood or plasma determinations).


Is EDTA natural?

Can you get EDTA naturally from foods? EDTA is a chemical that is added to certain foods and beverages to help them keep their colour and flavour. For instance, it is sometimes added to: Sodas.


Why is EDTA insoluble in water?

The carboxyl groups of EDTA are not dissociated at low pH. Undissociated carboxyls (COOH) have no charge because the hydrogen is covalently bound and therefore acid EDTA is almost insoluble in water. You may use Na-EDTA, which is soluble salt. Dissociated EDTA is ionic and thus water soluble.